mouse emx1 cre b6 129s2 emx1 tm1 cre krj j  (Jackson Laboratory)

Journal: iScience

Article Title: Genetic dissection of the role of Piga and Pgap2 in the embryonic mouse brain

doi: 10.1016/j.isci.2026.116299

Figure Lengend Snippet: Piga depletion from the forebrain causes mild cortical defects and hypomyelination (A) Genotype ratios of forebrain ablated Piga animals are similar to expected genotype ratios at P21 ( n = 62, Chi-square p = 0.406). Hematoxylin and eosin (H + E) images of (B) wild type, (C) Emx1 Cre/wt ; Piga flox/X female and (D) Emx1 Cre/wt ; Piga flox/Y male P21 forebrain sections ( n = 3 animals per genotype, scale bars, 1 mm). (E) Quantification of forebrain area in sections highlighted in images B and C ( n = 3 with each point representing an average from multiple sections for each animal, ANOVA: p = 0.472). Data represent individual values and the mean ± s.e.m. Immunostaining of myelin basic protein (MBP) (F–H), CUX1 (I–K) and CTIP2 (M–O), and EdU (Q–S) in wild type (F, I, M, and Q), Emx1 Cre/wt ; Piga flox/X female (G, J, N, and R), and Emx1 Cre/wt ; Piga flox/Y male (H, K, O, and S) P21 forebrain sections ( n = 4). Black arrows indicate MBP levels in the corpus callosum. Quantification of CUX1 (L, n = 4 ANOVA p < 0.001), CTIP2 (P, n = 4, ANOVA p < 0.001), and EdU positive cells distance from the VS (T, n = 4, ANOVA p < 0.001). Data represent individual cells and the black bar represents the mean ± s.e.m. Scale bar in F–H: 250 μm, I–K, M–O, Q–S: 50 μm.

Article Snippet: Mouse: Emx1-Cre ; B6.129S2- Emx1 tm1(cre)Krj /J , The Jackson Laboratory , RRID:IMSR_JAX:005628.

Techniques: Immunostaining

Journal: iScience

Article Title: Genetic dissection of the role of Piga and Pgap2 in the embryonic mouse brain

doi: 10.1016/j.isci.2026.116299

Figure Lengend Snippet: Pgap2 ablation in the forebrain and the cerebellum does not disrupt brain development (A) X-gal stain of Pgap2 null/wt P21 brain sections (scale bars, 1 mm) in the forebrain cortex (CX), striatum (ST), corpus callosum (CC), and (B) cerebellum (CB, scale bars, 500 μm). Black arrows pointing to the region of enhanced Pgap2 expression in the cortex and cerebellum. (C) Genotype ratios of forebrain ablation Pgap2 animals at P28 ( Emx1 Cre/wt ; Pgap2 flox/wt heterozygous and Emx1 Cre/wt ; Pgap2 flox/null mutant, n = 28, Chi-square p = 0.463). (D–F) Dorsal gross morphological images of wildtype, Emx1 Cre/wt ; Pgap2 flox/wt heterozygous, and Emx1 Cre/wt ; Pgap2 null/flox mutants (scale bars, 1 mm). Measurements of body weights (G, ANOVA p = 0.486), brain weights (H, ANOVA p = 0.823), and brain to body weight ratio (I, ANOVA p = 0.333) in wild type, Emx1 Cre ; Pgap2 flox/wt heterozygous and Emx1 Cre ; Pgap2 null/flox mutant (n = 3–9 animals per genotype). (J) Genotype ratios of cerebellar ablation of Pgap2 animals at P28 ( En1 Cre/wt ; Pgap2 flox/wt heterozygous and En1 Cre/wt ; Pgap2 flox/null mutant, Chi-square p = 0.889). (K–M) Caudal gross morphological images of the cerebellum in wild type, En1 Cre ; Pgap2 flox/wt heterozygous, and En1 Cre/wt ; Pgap2 null/flox mutants. (N–P) Measurements of body weights (N, ANOVA p = 0.614), cerebellar area (O, ANOVA p = 0.523), and cerebellar width (P, ANOVA p = 0.335) in wild type, En1 Cre/wt ; Pgap2 flox/wt heterozygous, and En1 Cre/wt ; Pgap2 flox/null mutants (n = 4–14 animals per genotype). Data represent individual values and the mean ± s.e.m.

Article Snippet: Mouse: Emx1-Cre ; B6.129S2- Emx1 tm1(cre)Krj /J , The Jackson Laboratory , RRID:IMSR_JAX:005628.

Techniques: Staining, Expressing, Mutagenesis

Journal: Cell reports

Article Title: Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development

doi: 10.1016/j.celrep.2025.115384

Figure Lengend Snippet:

Article Snippet: Mouse: Emx1-Cre , Jackson Laboratory , Strain#: 005628; RRID: IMSR_JAX:005628.

Techniques: Recombinant, Electron Microscopy, RNAscope, Isolation, Software, Microscopy